GPR17 Antibody (CL10474) - BSA Free

Immunohistochemistry-Paraffin: GPR17 Antibody (CL10474) [NBP3-07993] - Analysis in human cerebral cortex and kidney tissues using NBP3-07993 antibody. Corresponding GPR17 RNA-seq data are presented for the same tissues.

Western Blot: GPR17 Antibody (CL10474) [NBP3-07993] - Analysis in human cell line U-251 MG.

Immunohistochemistry-Paraffin: GPR17 Antibody (CL10474) [NBP3-07993] - Staining of human prostate shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: GPR17 Antibody (CL10474) [NBP3-07993] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of oligodendrocytes.

Immunohistochemistry-Paraffin: GPR17 Antibody (CL10474) [NBP3-07993] - Staining of human kidney shows no positivity in cells in tubules as expected.

Immunohistochemistry-Paraffin: GPR17 Antibody (CL10474) [NBP3-07993] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL10474
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

GPR17 Antibody (CL10474) - BSA Free Summary

• Description: Novus Biologicals Mouse GPR17 Antibody (CL10474) - BSA Free (NBP3-07993) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from Q13304, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPR17
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for GPR17 Antibody (CL10474) - BSA Free

• DKFZp686M18273
• G protein-coupled receptor 17
• GPR17
• G-protein coupled receptor 17
• P2Y-like Receptor
• R12
• UDP/CysLT Receptor
• uracil nucleotide/cysteinyl leukotriene receptor

Background

GPR17, a former orphan G-coupled protein is specifically activated by P2Y and CysLT endogenous ligands, leading to both adenylyl cyclase inhibition and intracellular calcium increases. In both rats and humans GPR17 expressed in the organs which suffer ischemic damage, like the brain, heart and kidneys. Studies by Ciana, et. al. have shown that inhibition of GPR17 reduces ischemic damage in a rat focal ischemia model. These results suggest GPR17 is the common molecular target mediating brain damage by nucleotides and CysLTs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR17