GNIP Antibody

Western Blot: GNIP Antibody [NB100-1229] - Staining of Human Skeletal Muscle lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: GNIP Antibody [NB100-1229] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GNIP Antibody Summary

• Immunogen: Peptide with sequence C-QAPAGPPWDITEA corresponding to C-Terminus according to NP_203128.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 ug/ml
• Application Notes: WB: Approx. 25-30 kDa band observed in human skeletal muscle lysate (predicted MW of 25 kDa band according to NP_203128). IHC-P: Human skeletal muscle shows pixulate staining in the fibres.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GNIP Antibody

• Glycogenin-interacting protein
• GNIPRING finger protein 90
• RNF90tripartite motif protein TRIM7
• tripartite motif containing 7
• tripartite motif-containing 7
• tripartite motif-containing protein 7

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1, a B-box type 2, and a coiled-coil region. The protein localizes to both the nucleus and the cytoplasm, and may represent a participant in the initiation of glycogen synthesis. Multiple transcript variants have been found for this gene, and some of them encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GNIP Antibody (NB100-1229)(1)

Publication using NB100-1229

• Skurat AV, Dietrich AD, Zhai L, Roach PJ. GNIP, a novel protein that binds and activates glycogenin, the self-glucosylating initiator of glycogen biosynthesis. J Biol Chem 2002-05-31 [PMID: 11916970]

Bioinformatics

• Gene Symbol: TRIM7
• Entrez:
  • 81786(Human)
• Uniprot:
  • NP_203128.(Human)