Recombinant Human GNGT1 His Protein

SDS-Page: GNGT1 Recombinant Protein [NBP2-51827] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human GNGT1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-71 of Human GNGT1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPVINIE DLTEKDKLKM EVDQLKKEVT LERMLVSKCC EEVRDYVEER SGEDPLVKGI PEDKNPFKEL KGGC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GNGT1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 10.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl, 1 mM EDTA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human GNGT1 His Protein

• GNG1
• guanine nucleotide binding protein (G protein), gamma transducing activitypolypeptide 1
• guanine nucleotide-binding protein G(T) subunit gamma-T1
• Transducin gamma chain

Background

GNGT1, also known as Guanine nucleotide-binding protein G(T) subunit gamma-T1, is a 74 amino acid that is 8 kDa, composed of 3 units, alpha, beta and gamma; found in the cell membrane; linked to 7-TM receptors; modulates or transduces in various transmembrane signaling systems; and its beta and gamma chains are requisite for the GTPase activity, for substitution of GDP by GTP, and for G protein-effector interaction. Studies on this protein has shown a relationship with fructose-1,6-bisphosphatase deficiency, retinitis pigmentosa, retinitis, and hypoxia. The GNGT1 protein has also shown an interaction with approx. 300 proteins including GNB1, GNB3, IKBKG, GNAS, and PLEKHB1 in several pathways including chemotaxis CXCR4 signaling pathway, translation regulation by Alpha-1 adrenergic receptors, development activation of ERK by Alpha-1 adrenergic receptors, cytoskeleton remodeling Role of PKA in cytoskeleton reorganization, development A1 receptor signaling, GPCR downstream signaling, transmission across chemical synapses, signaling by GPCR, G protein gated potassium channels, and neuronal system pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GNGT1