GNB1 Antibody

Western Blot: GNB1 Antibody [NB120-3433] - Analysis of Tr beta on sheep retinal extracts.

Product Discontinued

GNB1 Antibody Summary

• Immunogen: Synthetic Peptide: R(8) Q E A E Q L K N Q I R D A R K A C(25)
• Specificity: The NB120-3433 immunizing peptide corresponds to amino acid residues 8-25 from human Tr beta.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: WB: Detects an approx. 37 kDa protein representing Transducin beta from sheep retinal extract.

Reactivity Notes

Expected to cross-react with Rat and Amphibians due to sequence identity.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GNB1 Antibody

• beta subunit, signal-transducing proteins GS/GI
• G protein, beta-1 subunit
• guanine nucleotide binding protein (G protein), beta polypeptide 1
• guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1
• guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
• Transducin beta chain 1

Background

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GNB1 Antibody (NB120-3433)(1)

Publication using NB120-3433

• Morshedian A, Sendek G, Ng SY et al. Reproducibility of the rod photoreceptor response depends critically on concentration of the phosphodiesterase effector enzyme The Journal of neuroscience : the official journal of the Society for Neuroscience 2022-01-28 [PMID: 35091503]

Bioinformatics

• Gene Symbol: GNB1
• Entrez:
  • 2782(Human)