Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Colorimetric)

Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Colorimetric) - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 2.34 - 150 ng/mL
• Sensitivity: 1.4 ng/mL

Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Glutathione S-Transferase pi 1/GSTP1 in samples. No significant cross reactivity or interference between Human Glutathione S-Transferase pi 1/GSTP1 and analogues was observed.
• Standard Curve Range: 2.34 - 150 ng/mL
• Sensitivity: 1.4 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.96
• Intra-Assay: CV% < 5.11
• Spike Recovery: 85 - 107%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GSTP1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Colorimetric)

• deafness, X-linked 7
• DFN7
• EC 2.5.1.18
• FAEES3
• fatty acid ethyl ester synthase III
• glutathione S-transferase P
• Glutathione STransferase pi 1
• Glutathione S-Transferase pi 1
• GST class-pi
• GST3DFN7
• GSTP
• GSTP1
• GSTP1-1
• PI

Background

GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13 , is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (NBP3-25939). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GSTP1