Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) - Azide and BSA Free

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) [H00002950-M01] - Analysis of GSTP1 expression in transfected 293T cell line by GSTP1 monoclonal antibody (M01), clone 2G6-F6.Lane 1: GSTP1 transfected lysate (Predicted MW: 23.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) [H00002950-M01] - Analysis of monoclonal antibody to GSTP1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunoprecipitation: Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) [H00002950-M01] - Analysis of GSTP1 transfected lysate using anti-GSTP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GSTP1 MaxPab rabbit polyclonal antibody.

Sandwich ELISA Capture: Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) [H00002950-M01] - Detection limit for recombinant GST tagged Glutathione S-Transferase pi 1/GSTP1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP, ELISA
• Clone: 2G6-F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GSTP1 (AAH10915, 1 a.a. ~ 210 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPEFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYVSLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ
• Specificity: GSTP1 - glutathione S-transferase pi
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSTP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA Capture
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) - Azide and BSA Free

• deafness, X-linked 7
• DFN7
• EC 2.5.1.18
• FAEES3
• fatty acid ethyl ester synthase III
• glutathione S-transferase P
• Glutathione STransferase pi 1
• Glutathione S-Transferase pi 1
• GST class-pi
• GST3DFN7
• GSTP
• GSTP1
• GSTP1-1
• PI

Background

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione S-Transferase pi 1/GSTP1 Antibody (H00002950-M01)(3)

Publications using H00002950-M01

• Khabaz MN. Polymorphism of the glutathione S-transferase P1 gene (GST-pi) in breast carcinoma. Pol J Pathol. 2014-06-01 [PMID: 25119175]
• Kan CY, Wen VW, Pasquier E et al. Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization. Oncogene. 2012-02-06 [PMID: 22310292]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: GSTP1
• Entrez:
  • 2950(Human)
• OMIM:
  • 134660(Human)
• Uniprot:
  • AAH10915(Human)