Glutathione S-Transferase mu 1/GSTM1 Antibody

Western blot shows lysates of human kidney tissue, human adrenal gland tissue, human stomach tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Glutathione S-Transferase mu 1/GS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glutathione S-Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Glutathione S-Transferase mu 1/GSTM1 Antibody Summary

• Immunogen: E. coli-derived recombinant human Glutathione S‑Transferase mu 1/GS
  Met1-Lys218
  Accession # P09488
• Specificity: Detects human Glutathione S‑Transferase mu 1/GS in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GSTM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutathione S-Transferase mu 1/GSTM1 Antibody

• EC 2.5.1.18
• glutathione S-alkyltransferase
• glutathione S-aralkyltransferase
• glutathione S-aryltransferase
• glutathione S-transferase M1
• Glutathione STransferase mu 1
• Glutathione S-Transferase mu 1
• GST class-mu 1
• GST HB subunit 4
• GST1
• GSTM1
• GSTM1-1
• GSTM1a-1a
• GSTM1b-1b
• GTH4
• GTM1
• HB subunit 4
• H-B
• MGC26563
• MU
• MU-1
• S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).

1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
5. Mo, Z. et al. (2009) Prostate. 69:662.
6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSTM1
• Uniprot:
  • P09488()