Glutathione Peroxidase 5 Antibody (3B9) - Azide and BSA Free

Immunoprecipitation: Glutathione Peroxidase 5 Antibody (3B9) [H00002880-M02] - Analysis of GPX5 transfected lysate using anti-GPX5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GPX5 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: Glutathione Peroxidase 5 Antibody (3B9) [H00002880-M02] - Detection limit for recombinant GST tagged GPX5 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA, IP
• Clone: 3B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glutathione Peroxidase 5 Antibody (3B9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GPX5 (NP_001500, 122 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK
• Specificity: GPX5 - glutathione peroxidase 5 (epididymal androgen-related protein) (3B9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPX5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
• Application Notes: It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glutathione Peroxidase 5 Antibody (3B9) - Azide and BSA Free

• EC 1.11.1
• EC 1.11.1.9
• EGLP
• epididymal androgen-related protein
• epididymal secretory glutathione peroxidase
• Epididymis-specific glutathione peroxidase-like protein
• glutathione peroxidase 5 (epididymal androgen-related protein)
• Glutathione peroxidase 5
• GPx-5
• GSHPx-5

Background

Glutathione peroxidase (GPX)/glutathione reductase is part of the hydrogen peroxide scavenging system found within the epididymis in the mammalian male reproductive tract. GPX5 expression is epididymis-specific and the transcript is unique from other GPXs because it contains a deletion resulting in an mRNA that does not contain a selenocysteine (UGA) codon (an unusual amino acid present in other GPXs). This deletion also renders the mRNA incapable of encoding an active GPX isoenzyme. For this reason, GPX5 is selenium-independent and has very little activity towards hydrogen peroxide or organic hydroperoxides. GPX5, which is bound to the acrosome of sperm, may act to protect sperm from premature acrosome reaction in the epididymis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX5
• Entrez:
  • 2880(Human)
• Uniprot:
  • NP_001500(Human)