Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric)

Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) - Standarsd Curve Reference

ELISA: Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68015] - Samples were spiked with high concentrations of Mouse Glutathione Peroxidase 1/GPX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68015] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.50 - 4000 pg/mL
• Sensitivity: 37.50 pg/mL

Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse GPX1 in samples. No significant cross-reactivity or interference between Mouse GPX1 and analogues was observed.
• Standard Curve Range: 62.50 - 4000 pg/mL
• Sensitivity: 37.50 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.3
• Intra-Assay: CV% < 4.86
• Spike Recovery: 89-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GPX1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric)

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Glutathione Peroxidase 1/GPX1 ELISA Kit (NBP2-68015)(2)

Publications using NBP2-68015

• Yong Wang, Haiying Wang, Fengzhen Yang Barbaloin Treatment Contributes to the Rebalance of Glucose and Lipid Homeostasis of Gestational Diabetes Mellitus Mice Dose-Response 2020-12-29 [PMID: 33456413] (ELISA, Rat)
• Li Y, Li S, Li D Breviscapine Alleviates Cognitive Impairments Induced by Transient Cerebral Ischemia/Reperfusion through Its Anti-Inflammatory and Anti-Oxidant Properties in a Rat Model ACS chemical neuroscience 2020-12-16 [PMID: 33270442] (ELISA, Rat)

FAQs for Glutathione Peroxidase 1/GPX1 ELISA Kit (NBP2-68015). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GPX1