Glutathione Peroxidase 1/GPX1 Antibody

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Analysis of paraformaldehyde-fixed HeLa, using Glutathione Peroxidase 1 (NBP1-33620) antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Rat liver lysate. 12% SDS-PAGE gel, GPX1 antibody dilution: 1:10000.

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - A. 30 ug GL261 whole cell lysate, B. 30 ug C8D30 whole cell lysate, C. 30 ug NIH-3T3 whole cell lysate, D. 30 ug Raw264. 7 whole cell lysate, E. 30 ug C2C12 whole cell lysate. 12% SDS-PAGE.

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Alcohol feeding did not change the expression of GPX1 in mouse liver. Image from verified customer review.

Product Discontinued

Glutathione Peroxidase 1/GPX1 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Application Notes: Use in immunoblotting reported in scientific literature (PMID: 27575873). Glutathione Peroxidase 1/GPX1 antibody validated for WB from a verified customer review.
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)(7)

Publications using NBP1-33620

• Palomera-Avalos V, Grinan-Ferre C, Izquierdo V et al. Resveratrol modulates response against acute inflammatory stimuli in aged mouse brain. Exp. Gerontol. 2017-11-24 [PMID: 29174969] (Mouse)
• Sun Q, Zhang W, Zhong W et al. Dietary Fisetin Supplementation Protects Against Alcohol-Induced Liver Injury in Mice. Alcohol Clin Exp Res 2016-08-30 [PMID: 27575873] (IB)
• Yew M, Koh R, Chye S et al. Edible birds nest improves motor behavior and protects dopaminergic neuron against oxidative and nitrosative stress in Parkinsons disease mouse model Journal of Functional Foods 2018-09-01 (IF/IHC, Mouse)
• Foisy-SauvE M, Ahmarani L, Delvin E et al. Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells Nutrients 2020-04-22 [PMID: 32331475] (WB, Human)
• Kumar A, Vaish M, Karuppagounder SS, Gazaryan I HIF1 alpha stability in hypoxia is not oxidant-initiated but can be tuned by redox regulation of the proteasome bioRxiv 2020-01-01 (IP, Mouse)
• Puigoriol-Illamola D, Martinez-Damas M, Grinan-Ferre C, Pallas M Chronic Mild Stress Modified Epigenetic Mechanisms Leading to Accelerated Senescence and Impaired Cognitive Performance in Mice Preprints 2020-01-29 [PMID: 32050516] (WB, Mouse)
• Grinan-FerrE C, Marsal-Garcia L et al. Pharmacological inhibition of G9a/GLP restores cognition and reduces oxidative stress, neuroinflammation and beta-Amyloid plaques in an early-onset Alzheimer's disease mouse model. Aging (Albany NY) 2019-04-12 [PMID: 31804189] (WB, Mouse)

Review for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Wenliang Zhang WB Mouse 02/12/2018

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: 40135

Bioinformatics

• Gene Symbol: GPX1