Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Analysis of paraformaldehyde-fixed HeLa, using Glutathione Peroxidase 1 (NBP1-33620) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Rat liver lysate/extract 12 % SDS-PAGE gel,GPX1 antibody dilution: 1:10000.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - A. 30 ug GL261 whole cell lysate/extract lysate/extract, B. 30 ug C8D30 whole cell lysate/extract, C. 30 ug NIH-3T3 whole cell lysate/extract. D. 30 ...read more
22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Immunogen affinity purified
Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody
Cellular glutathione peroxidase
Glutathione Peroxidase 1
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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