Glutathione Peroxidase 1/GPX1 Antibody

Images

 
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Alcohol feeding did not change the expression of GPX1 in mouse liver. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Analysis of paraformaldehyde-fixed HeLa, using Glutathione Peroxidase 1 (NBP1-33620) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Rat liver lysate. 12% SDS-PAGE gel, GPX1 antibody dilution: 1:10000.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - A. 30 ug GL261 whole cell lysate, B. 30 ug C8D30 whole cell lysate, C. 30 ug NIH-3T3 whole cell lysate, D. 30 ug Raw264. 7 whole cell lysate, E. 30 ug ...read more
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glutathione Peroxidase 1/GPX1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Glutathione Peroxidase 1/GPX1 Lysate (NBP2-66091)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-33620 in the following applications:

Publications
Read Publications using
NBP1-33620 in the following applications:

  • IB
    1 publication

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody

  • Cellular glutathione peroxidase
  • CGPx
  • EC 1.11.1
  • EC 1.11.1.9
  • Glutathione Peroxidase 1
  • GPX1
  • GPx-1
  • GSHPX1
  • GSHPx-1
  • MGC14399
  • MGC88245

Background

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NB100-1992
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gt, GP, Ha, Mk, Rb, Sh, Sq, Xp
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
MAB5457
Species: Hu, Mu, Rt
Applications: WB
NBP1-06398
Species: Hu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-86614
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-55298
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-24940
Species: Hu, Ye
Applications: WB
NB100-1888
Species: Hu, Mu, Rt
Applications: WB, IHC, PEP-ELISA
NBP2-20619
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF5724
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP1-32822
Species: Hu, Mu, Rt, Av, Ce
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-52983
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33620
Species: Hu, Mu, Rt, Pm, RM
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IB.


Filter By Application
IB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-33620:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Glutathione Peroxidase 1/GPX1 NBP1-33620
Enlarge
reviewed by:
Wenliang Zhang
WB Mouse 02/12/2018
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
Lot40135

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Glutathione Peroxidase 1/GPX1 Products

Bioinformatics Tool for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)

Discover related pathways, diseases and genes to Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)

Discover more about diseases related to Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620).
 

Pathways for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)

View related products by pathway.

PTMs for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)

Learn more about PTMs related to Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620).

Blogs on Glutathione Peroxidase 1/GPX1

There are no specific blogs for Glutathione Peroxidase 1/GPX1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Wenliang Zhang
02/12/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol GPX1
Entrez