Glutathione Peroxidase 1/GPX1 Antibody

Images

 
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX1 antibody diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Analysis of paraformaldehyde-fixed HeLa, using Glutathione Peroxidase 1 (NBP1-33620) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Rat liver lysate. 12% SDS-PAGE gel, GPX1 antibody dilution: 1:10000.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - A. 30 ug GL261 whole cell lysate, B. 30 ug C8D30 whole cell lysate, C. 30 ug NIH-3T3 whole cell lysate, D. 30 ug Raw264. 7 whole cell lysate, E. 30 ug ...read more
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Alcohol feeding did not change the expression of GPX1 in mouse liver. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Al, Pm, RMSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glutathione Peroxidase 1/GPX1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Use in immunoblotting reported in scientific literature (PMID: 27575873). Glutathione Peroxidase 1/GPX1 antibody validated for WB from a verified customer review.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Glutathione Peroxidase 1/GPX1 Knockout HeLa Cell Lysate
Glutathione Peroxidase 1/GPX1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-33620 in the following applications:

Publications
Read Publications using
NBP1-33620 in the following applications:

  • IB
    1 publication
  • IHC
    1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody

  • Cellular glutathione peroxidase
  • CGPx
  • EC 1.11.1
  • EC 1.11.1.9
  • Glutathione Peroxidase 1
  • GPX1
  • GPx-1
  • GSHPX1
  • GSHPx-1
  • MGC14399
  • MGC88245

Background

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IB, IHC, IP, WB.


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Showing Publications 1 - 5 of 5.
Publications using NBP1-33620 Applications Species
Foisy-SauvE M, Ahmarani L, Delvin E et al. Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells Nutrients Apr 22 2020 [PMID: 32331475] (WB, Human) WB Human
Palomera-Avalos V, Grinan-Ferre C, Izquierdo V et al. Resveratrol modulates response against acute inflammatory stimuli in aged mouse brain. Exp. Gerontol. 2017 Nov 24 [PMID: 29174969] (Mouse) Mouse
Sun Q, Zhang W, Zhong W et al. Dietary Fisetin Supplementation Protects Against Alcohol-Induced Liver Injury in Mice. Alcohol. Clin. Exp. Res. Aug 30 2016 [PMID: 27575873] (IB) IB
Kumar A, Vaish M, Karuppagounder SS, Gazaryan I HIF1 alpha stability in hypoxia is not oxidant-initiated but can be tuned by redox regulation of the proteasome bioRxiv Jan 1 2020 (IP, Mouse) IP Mouse
Yew M, Koh R, Chye S et al. Edible birds nest improves motor behavior and protects dopaminergic neuron against oxidative and nitrosative stress in Parkinsons disease mouse model Journal of Functional Foods Sep 1 2018 (IHC, Mouse) IHC Mouse

Review for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-33620:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Glutathione Peroxidase 1/GPX1 NBP1-33620
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reviewed by:
Wenliang Zhang
WB Mouse 02/12/2018
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Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
Lot40135

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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4
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1
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Wenliang Zhang
02/12/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol GPX1