Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX1 antibody diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Analysis of paraformaldehyde-fixed HeLa, using Glutathione Peroxidase 1 (NBP1-33620) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Rat liver lysate. 12% SDS-PAGE gel, GPX1 antibody dilution: 1:10000.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - A. 30 ug GL261 whole cell lysate, B. 30 ug C8D30 whole cell lysate, C. 30 ug NIH-3T3 whole cell lysate, D. 30 ug Raw264. 7 whole cell lysate, E. 30 ug ...read more
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP1-33620] - Alcohol feeding did not change the expression of GPX1 in mouse liver. Image from verified customer review.
Use in immunoblotting reported in scientific literature (PMID: 27575873). Glutathione Peroxidase 1/GPX1 antibody validated for WB from a verified customer review.
22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 20% Glycerol (pH7).
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody
Cellular glutathione peroxidase
Glutathione Peroxidase 1
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620)(5)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 4 applications: IB, IHC, IP, WB.
Kumar A, Vaish M, Karuppagounder SS, Gazaryan I HIF1 alpha stability in hypoxia is not oxidant-initiated but can be tuned by redox regulation of the proteasome bioRxiv Jan 1 2020 (IP, Mouse)
Yew M, Koh R, Chye S et al. Edible birds nest improves motor behavior and protects dopaminergic neuron against oxidative and nitrosative stress in Parkinsons disease mouse model Journal of Functional Foods Sep 1 2018 (IHC, Mouse)
Review for Glutathione Peroxidase 1/GPX1 Antibody (NBP1-33620) (1)
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.