Glutaredoxin 3/GLRX3 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

Glutaredoxin 3/GLRX3 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Glutaredoxin 3/GLRX3
  Asn126-Lys294
  Accession # O76003
• Specificity: Detects human, mouse and rat Glutaredoxin 3/GLRX3 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutaredoxin 3/GLRX3 Antibody [Alexa Fluor® 488]

• FLJ11864
• GLRX3
• GLRX4
• Glutaredoxin 3
• glutaredoxin 4
• glutaredoxin 5
• GRX3
• GRX4
• PICOT
• PICOTbA500G10.4
• PKC-interacting cousin of thioredoxin
• PKCq-interacting protein
• thioredoxin-like 2
• TXNL2
• TXNL3

Background

GLRX3 (Glutaredoxin-3; also PICOT, PKC-theta-interacting protein, and Thioredoxin-like protein 2/TXNL2) is a 37-40 kDa member of the multidomain subgroup, monothiol glutaredoxin group of glutaredoxin/GTX proteins. It has widespread expression, but is not found ubiquitously. Cells known to express cytosolic GLRX3 are principally epithelial in type, and include breast and adrenal epithelium, pancreatic exocrine gland epithelium, and proximal tubule renal epithelium. Cardiac muscle may also expresses GLRX3. The function of GLRX3 is not clear. By inference, it is suggested to be involved in cell proliferation, and has also been associated with both PKC-theta regulation and MLP/muscle LIM protein-mediated cardiac contractility. Human GLRX3 is 335 amino acids (aa) in length. It contains an N-terminal thioredoxin-like domain (aa 2-117) that is followed by two PICOT homology domains (aa 144-236 and 237-335). The thioredoxin domain does not possess a typical disulfide, and it is suggested that this domain does not demonstrate redox activity. The PICOT domains will interact with intracellular proteins. GLRX3 exists as both a monomer and homodimer, with the homodimer incorporating two Fe/S clusters into their PICOT domains. These clusters serve as redox sensors within the cell. Over aa 126-294, human GLRX3 shares 98% aa sequence identity with mouse GLRX3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.