HAF016). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Glutaredoxin 3/GLRX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7560) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7560-SP | |||
HAF018). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Glutaredoxin 3/GLRX3 Monoclonal Antibody (Catalog # MAB7560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7560-SP | |||
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in ductal cells. View our protocol for ![Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/TXNL2-Antibody-Western-Blot-NBP2-20767-img0002.jpg "Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/TXNL2-Antibody-Western-Blot-NBP2-20767-img0001.jpg)
![Immunoprecipitation: Glutaredoxin 3/GLRX3 Antibody [NBP2-20767] - TXNL2 protein from 293T whole cell extracts using 5 ug of TXNL2 antibody [N2C3]. Western blot analysis was performed using TXNL2 antibody [N2C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Glutaredoxin-3-GLRX3-Antibody-Immunoprecipitation-NBP2-20767-img0005.jpg)
![TXNL2 antibody [N2C3] detects TXNL2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. <br>Sample: Paraffin-embedded human breast carcinoma. <br>TXNL2 antibody [N2C3] (NBP2-20767) dilution: 1:200.<br> br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-20767_rabbit-polyclonal-glutaredoxin-3-glrx3-antibody-immunohistochemistry-2611202518204712.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
![Western Blot: Glutaredoxin 3/GLRX3 Antibody (4B5-2A8) [H00010539-M01] - GLRX3 monoclonal antibody (M01), clone 4B5-2A8 Analysis of GLRX3 expression in Hela.](http://images.novusbio.com/fullsize/TXNL2-Antibody-4B5-2A8-Western-Blot-H00010539-M01-img0002.jpg "Western Blot: Glutaredoxin 3/GLRX3 Antibody (4B5-2A8) [H00010539-M01] - GLRX3 monoclonal antibody (M01), clone 4B5-2A8 Analysis of GLRX3 expression in Hela.")
![Immunoprecipitation:Glutaredoxin 3/GLRX3 AntibodyNBP3-29814] -Detection of human GLRX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLRX3 antibody NBP3-29814 (lot NBP3-29814-1) used for IP at 6 µg per reaction. GLRX3 was also immunoprecipitated by rabbit anti-GLRX3.. For blotting immunoprecipitated GLRX3, NBP3-29814 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29814_rabbit-glutaredoxin-3-glrx3-pab-301220242036223.jpg "Immunoprecipitation:Glutaredoxin 3/GLRX3 AntibodyNBP3-29814] -Detection of human GLRX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLRX3 antibody NBP3-29814 (lot NBP3-29814-1) used for IP at 6 µg per reaction. GLRX3 was also immunoprecipitated by rabbit anti-GLRX3.. For blotting immunoprecipitated GLRX3, NBP3-29814 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot:[NBP3-30161]-Detection of human and mouse GLRX3 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GLRX3 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-30161_rabbit-glutaredoxin-3-glrx3-pab-2312025128870.jpg "Western Blot:[NBP3-30161]-Detection of human and mouse GLRX3 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GLRX3 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunoprecipitation:Glutaredoxin 3/GLRX3 AntibodyNBP3-29977] -Detection of human GLRX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLRX3 antibody NBP3-29977 (lot NBP3-29977-1) used for IP at 6 µg per reaction. GLRX3 was also immunoprecipitated by rabbit anti-GLRX3.. For blotting immunoprecipitated GLRX3, NBP3-29977 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29977_rabbit-glutaredoxin-3-glrx3-pab-301220241928132.jpg "Immunoprecipitation:Glutaredoxin 3/GLRX3 AntibodyNBP3-29977] -Detection of human GLRX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLRX3 antibody NBP3-29977 (lot NBP3-29977-1) used for IP at 6 µg per reaction. GLRX3 was also immunoprecipitated by rabbit anti-GLRX3.. For blotting immunoprecipitated GLRX3, NBP3-29977 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20766] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/TXNL2-Antibody-Western-Blot-NBP2-20766-img0001.jpg "Western Blot: Glutaredoxin 3/GLRX3 Antibody [NBP2-20766] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.")
