Recombinant Human Glutaredoxin 2 Protein

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SDS-Page: Recombinant Human Glutaredoxin 2 Protein [NBP1-30299] - 15% SDS-Page (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Glutaredoxin 2 Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 20-164 of Human Glutaredoxin 2

Source: E.coli

Amino Acid Sequence: MSAGWLDRAA GAAGAAAAAA SGMESNTSSS LENLATAPVN QIQETISDNC VVIFSKTSCS YCTMAKKLFH DMNVNYKVVE LDLLEYGNQF QDALYKMTGE RTVPRIFVNG TFIGGATDTH RLHKEGKLLP LVHQCYLKKS KRKEFQLEHH HHHH

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GLRX2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 mM PMSF, 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Glutaredoxin 2 Protein

  • bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2)
  • glutaredoxin 2
  • glutaredoxin-2, mitochondrial
  • GRX2bA101E13.1

Background

Glutaredoxin-2, as known as GRX2, multifunctional enzyme with glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase(GST) activity. This protein is localized both in the mitochondria and nucleus. It has anti-apoptotic activity by preventing cardiolipin oxidation and cytochrome c release. GRX2 exhibited 36% identity with GRX1 and had a disulfide active center containing the Cys-Ser-Tyr-Cys motif. Recombinant human Glutaredoxin 2, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GLRX2