Glutaredoxin 1/GLRX1 Antibody

Western Blot: Glutaredoxin 1 Antibody [NB110-81753] - Detection of GLRX in Jurkat whole cell lysate using NB110-81753.

Product Discontinued

Glutaredoxin 1/GLRX1 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human Glutaredoxin 1 (within residues 40-100). [Swiss-Prot# P35754]
• Localization: Cytoplasm.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Zebrafish (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLRX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
• Application Notes: This Glutaredoxin 1 antibody is useful for Western blot where a band is seen ~14 kDa. This antibody is not useful for ICC/IF. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Glutaredoxin 1/GLRX1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutaredoxin 1/GLRX1 Antibody

• GLRX
• glutaredoxin (thioltransferase)
• Glutaredoxin 1
• glutaredoxin-1
• GRXGRX1
• MGC117407
• Thioltransferase
• Thioltransferase-1
• TTase-1

Background

Glutaredoxins (GRXs) are one of thioredoxin superfamily members of thiol/disulfide exchange catalysts, and hence known as thioltransferases serving as reductants of protein SG mixed disulfides providing reducing equivalents to ribonucleotide reductase. Among two mammalian GRXs, GRX1 (Glutaredoxin 1) localizes in the cytosol primarily to mitochondria and to lesser extent in nucleus, whereas GRX2 has nuclear as well as mitochondrial isoforms. GRX1 is a relatively abundant, small ~12 kD cytosolic enzyme that regulates protein mixed disulfides through their reduction by GSH (S-glutathiolated proteins; R-SSG) and its functions depends upon GSH presence. When GRX1 deglutathionylates an S-glutathionylated substrate, a GRX1-SSG intermediate complex is formed, and the free GSH/glutathione reductase (GR) reduces the intermediate complex with NADPH to NADP+ and "reload" GRX1 to continue the catalysis. Unlike thioredoxin 1, GRX1 does not catalyze the reduction of sulfenic acid or intra-/inter-molecular disulfides, thereby efficiently catalyzing the reduction of R-SSG in the presence of NADPH and GR. Glrx1 is upregulated by several cytokines including IL-4, IL-6, IL-13 etc. and its expression is decreased by TGF-beta1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLRX
• Entrez:
  • 2745(Human)
• Uniprot:
  • P35754()