Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody - Azide and BSA Free

Western Blot: Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody [H00002650-B01P] - Analysis of GCNT1 expression in transfected 293T cell line by GCNT1 polyclonal antibody. Lane1:GCNT1 transfected lysate(47.08 KDa). Lane2:Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: GCNT1 (NP_001481.2, 1 a.a. - 428 a.a.) full-length human protein. MLRTLLRRRLFSYPTKYYFMVLVLSLITFSVLRIHQKPEFVSVRHLELAGENPSSDINCTKVLQGDVNEIQKVKLEILTVKFKKRPRWTPDDYINMTSDCSSFIKRRKYIVEPLSKEEAEFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANWKYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLETPLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAVARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWMLRKHHLFANKFDVDVDLFAIQCLDEHLRHKALETLKH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GCNT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is useful for WB. ICC/IF usage was reported in scientific literature.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody - Azide and BSA Free

• beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase
• beta-1,6-N-acetylglucosaminyltransferase
• C2GNT1
• C2GNTbeta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N--acetylglucosaminyltransferase
• C2GNT-L
• core 2 beta1,6 N-acetylglucosaminyltransferase-I
• core 2 beta-1,6-N-acetylglucosaminyltransferase I
• core 2 branching enzyme
• Core 2 GNT
• Core 2-branching enzyme
• core2-GlcNAc-transferase
• EC 2.4.1.102
• G6NT
• GCNT1
• glucosaminyl (N-acetyl) transferase 1, core 2
• glucosaminyl (N-acetyl) transferase 1, core 2(beta-1,6-N-acetylglucosaminyltransferase)
• MGC126335
• MGC126336
• NACGT2
• NAGCT2

Background

This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody (H00002650-B01P)(3)

Publications using H00002650-B01P

• Petrosyan Armen, Holzapfel Melissa S, Muirhead David E, Cheng Pi-Wan. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res. 2014-08-01 [PMID: 25086069]
• Petrosyan A, Cheng PW. Golgi fragmentation induced by heat shock or inhibition of heat shock proteins is mediated by non-muscle myosin IIA via its interaction with glycosyltransferases. Cell Stress Chaperones. 2013-08-30 [PMID: 23990450]
• Ali MF, Chachadi VB, Petrosyan A, Cheng PW. Golgi phosphoprotein 3 determines cell binding properties under dynamic flow by controlling Golgi localization of Core 2 N-acetylglucosaminyltransferase 1 J Biol Chem 2012-10-01 [PMID: 23027862] (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: GCNT1
• Uniprot:
  • NP_001481.2(Human)