GIT2 Antibody (M1)

Images

 
Western Blot: GIT2 Antibody (M1) [H00009815-M03] - Analysis of GIT2 expression in transfected 293T cell line by GIT2 monoclonal antibody (M03), clone M1. Lane 1: GIT2 transfected lysate (Predicted MW: 52.6 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: GIT2 Antibody (M1) [H00009815-M03] - Analysis of monoclonal antibody to GIT2 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: GIT2 Antibody (M1) [H00009815-M03] - Analysis of GIT2 transfected lysate using anti-GIT2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GIT2 rabbit polyclonal antibody.
ELISA: GIT2 Antibody (M1) [H00009815-M03] - Detection limit for recombinant GST tagged GIT2 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GIT2 Antibody (M1) Summary

Immunogen
GIT2 (AAH01379, 1 a.a. ~ 472 a.a) full length recombinant protein with GST tag.MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADS
Specificity
GIT2 - G protein-coupled receptor kinase interactor 2
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GIT2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GIT2 Antibody (M1)

  • ARF GAP GIT2
  • ARF GTPase-activating protein GIT2
  • CAT2
  • CAT-2
  • cool-associated, tyrosine phosphorylated protein 2
  • Cool-interacting tyrosine-phosphorylated protein 2
  • DKFZp686G01261
  • G protein-coupled receptor kinase interacting ArfGAP 2
  • G protein-coupled receptor kinase interactor 2
  • G protein-coupled receptor kinase-interactor 2
  • KIAA0148GRK-interacting protein 2
  • MGC760

Background

This gene encodes a member of the GIT protein family. GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. This gene undergoes extensive alternative splicing; although ten transcript variants have been described, the full length sequence has been determined for only four variants. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GIT2
Entrez
OMIM
Uniprot