GIT2 Antibody (1B2) - Azide and BSA Free

Western Blot: GIT2 Antibody (1B2) [H00009815-M04] - Analysis of GIT2 expression in transfected 293T cell line by GIT2 monoclonal antibody (M04), clone 1B2. Lane 1: GIT2 transfected lysate (Predicted MW: 52.6 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GIT2 Antibody (1B2) - Azide and BSA Free Summary

• Immunogen: GIT2 (NP_055591, 401 a.a. ~ 498 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TDLETTASKTNRQKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHA
• Specificity: Reacts with G protein-coupled receptor kinase interacting ArfGAP 2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GIT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in western blot and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GIT2 Antibody (1B2) - Azide and BSA Free

• ARF GAP GIT2
• ARF GTPase-activating protein GIT2
• CAT2
• CAT-2
• cool-associated, tyrosine phosphorylated protein 2
• Cool-interacting tyrosine-phosphorylated protein 2
• DKFZp686G01261
• G protein-coupled receptor kinase interacting ArfGAP 2
• G protein-coupled receptor kinase interactor 2
• G protein-coupled receptor kinase-interactor 2
• KIAA0148GRK-interacting protein 2
• MGC760

Background

This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GIT2
• Entrez:
  • 9815(Human)
• Uniprot:
  • NP_055591(Human)