GHRHR Antibody (1037308) [Unconjugated]

Western blot shows lysates of PC‑3 human prostate cancer cell line, LoVo human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 4 µg/mL of Mouse Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107352) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GHRHR at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 1037308
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GHRHR Antibody (1037308) [Unconjugated] Summary

• Immunogen: Human GHRHR synthetic peptide
  Accession # Q02643
• Specificity: Detects human GHRHR in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 4 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GHRHR Antibody (1037308) [Unconjugated]

• GHRFR
• GHRH receptor
• GHRHR
• GRF receptor
• GRFR
• growth hormone releasing hormone receptor
• Growth hormone-releasing factor receptor
• growth hormone-releasing hormone receptor
• IGHD1B
• Little

Background

GHRHR is a GPCR that binds growth hormone-releasing hormone that is expressed in the pituitary.  Significant amounts of GHRHR are also found in the hypothalamus, kidney, and placenta.  GHRH regulates the secretion of growth hormone that controls the metabolism and growth of every tissue through its binding with GHRHR.  Malfunction in GHRHR signaling is associated with abnormal growth.  GHRHR is a therapeutic target against dwarfism, gigantism, lipodystrophy, and certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q02643(Human)