GGPS1 Overexpression Lysate

Western Blot: GGPS1 Overexpression Lysate (Adult Normal) [NBL1-11058] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GGPS1.

• Reactivity: Hu
• Applications: WB

GGPS1 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC202699 and based on accession number NM_004837. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens geranylgeranyl diphosphate synthase 1 (GGPS1), transcript variant 1, mRNA.
• Gene: GGPS1

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 34.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GGPS1 Overexpression Lysate

• 6E)-farnesyl diphosphate synthase
• dimethylallyltranstransferase
• EC 2.5.1.1
• EC 2.5.1.10
• EC 2.5.1.29
• Farnesyl diphosphate synthase
• Farnesyltranstransferase
• geranylgeranyl diphosphate synthase 1
• Geranylgeranyl diphosphate synthase
• geranylgeranyl pyrophosphate synthase
• geranyltranstransferase
• GGPP synthase
• GGPPS
• GGPPS1farnesyltranstransferase
• GGPPSase

Background

This gene is a member of the prenyltransferase family and encodes a protein with geranylgeranyl diphosphate (GGPP) synthase activity. The enzyme catalyzes the synthesis of GGPP from farnesyl diphosphate and isopentenyl diphosphate. GGPP is an important molecule responsible for the C20-prenylation of proteins and for the regulation of a nuclear hormone receptor. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GGPS1