GGA1 Antibody (1G8) - Azide and BSA Free

Western Blot: GGA1 Antibody (1G8) [H00026088-M02] - Analysis of GGA1 expression in transfected 293T cell line by GGA1 monoclonal antibody (M02), clone 1G8.Lane 1: GGA1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.

Western Blot: GGA1 Antibody (1G8) [H00026088-M02] - Analysis of GGA1 over-expressed 293 cell line, cotransfected with GGA1 Validated Chimera RNAi ( Cat # H00026088-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GGA1 monoclonal antibody (M02), clone 1G8 (Cat # H00026088-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: GGA1 Antibody (1G8) [H00026088-M02] - Detection limit for recombinant GST tagged GGA1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 1G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GGA1 Antibody (1G8) - Azide and BSA Free Summary

• Immunogen: GGA1 (AAH29388, 1 a.a. ~ 89 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVRRGEATIRPPPCDDTKGGQD
• Specificity: GGA1 - golgi associated, gamma adaptin ear containing, ARF binding protein 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GGA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GGA1 Antibody (1G8) - Azide and BSA Free

• ADP-ribosylation factor binding protein 1
• ADP-ribosylation factor-binding protein GGA1
• gamma adaptin ear containing, ARF binding protein 1
• gamma-adaptin related protein 1
• Gamma-adaptin-related protein 1
• golgi-associated, gamma adaptin ear containing, ARF binding protein 1
• Golgi-localized, gamma ear-containing, ARF-binding protein 1

Background

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GGA1
• Entrez:
  • 26088(Human)
• OMIM:
  • 606004(Human)
• Uniprot:
  • AAH29388(Human)