Gephyrin/GPHN Antibody (JG34-47)

Western Blot: Gephyrin/GPHN Antibody (JG34-47) [NBP2-75504] - Analysis of Gephyrin on SK-Br-3 cell (1) and rat kidney tissue (2) lysate using anti-Gephyrin antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (JG34-47) [NBP2-75504] - Analysis of paraffin-embedded mouse brain tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (JG34-47) [NBP2-75504] - Analysis of paraffin-embedded human kidney tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Product Discontinued

Gephyrin/GPHN Antibody (JG34-47) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Gephyrin/GPHN aa 5-95 / 736. (SwissProt: Q9NQX3 Human; SwissProt: Q8BUV3 Mouse; SwissProt: Q03555 Rat)
• Localization: Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPHN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Gephyrin/GPHN Antibody (JG34-47)

• GEPH
• Gephyrin
• GPH
• GPHN
• GPHRYN
• KIAA1385GPHGEPH

Background

The Gephyrin (GPHN) gene encodes a neuronal assembly protein that exists in isoform 1 (736 amino acids long, 79 kDA) or isoform 2 (769 amino acids in length, approximately 83 kDA). This protein anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton through high affinity binding to a receptor subunit domain and tublin dimers. The protein coded by GPHN is also necessary for molybdenum biosynthesis. The GPHN gene has been investigated in relation to many diseases such as leukemia, cervicitis, tuberculosis, alzheimer's disease, anorexia nervosa, huntington's disease, retinitis, and herpes zoster. The GPHN gene interacts with genes DYNLL1, PFN1, PRPF4, ENAH, and SLX4 to participate in pathways that regulate metabolism of vitamins and cofactors as well as molybdenum cofactor biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPHN