Gephyrin/GPHN Antibody (807423) [Alexa Fluor® 532]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 807423
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Gephyrin/GPHN Antibody (807423) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Gephyrin/GPHN
  Ala2-Ser188
  Accession # Q9NQX3
• Specificity: Detects human Gephyrin/GPHN in direct ELISAs and human and mouse Gephyrin/GPHN Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gephyrin/GPHN Antibody (807423) [Alexa Fluor® 532]

• GEPH
• Gephyrin
• GPH
• GPHN
• GPHRYN
• KIAA1385GPHGEPH

Background

Gephyrin (GPHN, from gamma epsilon φυρ alpha , the Greek word for bridge; also known as molybdopterin adenyltransferase) is a 90-94 kDa member of both the MoaB and MoeA families of proteins. It has limited expression, being found in hepatocytes and neurons. In neurons, gephyrin is a postsynaptic density cytoplasmic protein that interacts with the subunits of glycine and GABA-A receptors. This interaction is mediated by the ability of gephyrin to form hexameric lattices that act as scaffolds that link the subunits of the two inhibitory receptors to the cell's underlying tubulin network. In addition, the gephyrin lattice may also interact with NLG-1 and -2, suggesting a role for gephyrin in the regulation of glutamatergic synapses. Gephyrin is also known to participate in the synthesis of molybdenum cofactor. This cofactor binds molybdenum, making it available to sulfite and aldehyde reductase. Human gephyrin is 736 amino acids (aa) in length. It contains a 181 aa N-terminal G-domain that possesses MPT Mo-transferase activity (aa 14-166) and a homotrimer interface motif, followed by a 420 aa C-terminal region (aa 319-738) that contains an MPT adenyltransferase domain (aa 326-736) with an embedded homodimerization motif. There are at least five utilized Ser/Thr phosphorylation sites. Multiple splice variants exist, and represent peptide insertions at multiple sites. There is a 13 aa insertion after Glu98, a 33 aa insertion after Ser243, and 15, 19, 21 and 24 aa insertions after Lys288. Over aa 2-188, human and mouse Gephyrin are identical in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.