GCNT3 Antibody

Western Blot: GCNT3 Antibody [NB100-93401] - HEK293 overexpressing GCNT3 and probed with (mock transfection in first lane).

Western Blot: GCNT3 Antibody [NB100-93401] - Analysis of GCNT3 (aa 410 to 422) in Human Duodenum lysate (35ug protein in RIPA buffer) using this antibody at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GCNT3 Antibody Summary

• Description: Novus Biologicals Goat GCNT3 Antibody (NB100-93401) is a polyclonal antibody validated for use in WB and ELISA. Anti-GCNT3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NKFDPKVDDNALQ corresponding to internal region (near C-Terminus) according to NP_004742.1.
• Epitope: C-NKFDPKVDDNALQ
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GCNT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 55-60 kDa band observed in human duodenum lysates (calculated MW of 50.9 kDa band according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  GCNT3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GCNT3 Antibody

• beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3
• beta1,6GlcNAc-transferase
• beta1,6-N-acetylglucosaminyltransferase-M
• C2/4GnTGNTM
• C24GNT
• C2GnT2
• C2GNTM
• C2GnT-M
• C2GnT-mucin type
• Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
• EC 2.4.1.102
• EC 2.4.1.150
• glucosaminyl (N-acetyl) transferase 3, mucin type
• hC2GnT-M
• mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

Background

This enzyme catalyzes O-glycan branch synthesis of the core 2 and core 4 type in mucins and controls expression of core 2 branched oligosaccharides and I antigens on the cell surface. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCNT3 Antibody (NB100-93401)(1)

Publication using NB100-93401

• Schwientek T, Yeh JC, Levery SB et al. Control of O-glycan branch formation. Molecular cloning and characterization of a novel thymus-associated core 2 beta1, 6-n-acetylglucosaminyltransferase. J Biol Chem 2000-04-14 [PMID: 10753916]

Bioinformatics

• Gene Symbol: GCNT3
• Entrez:
  • 9245(Human)
  • 72077(Mouse)
  • 286976(Rat)
• Uniprot:
  • NP_004742.1(Human)