GCNT3 Antibody

Western Blot: GCNT3 Antibody [NB100-93400] - HEK293 overexpressing GCNT3 and probed with (mock transfection in first lane).

Western Blot: GCNT3 Antibody [NB100-93400] - (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GCNT3 Antibody Summary

• Immunogen: Peptide with sequence C-RDTLHLTNKKKD corresponding to internal region according to NP_004742.1.
• Epitope: C-RDTLHLTNKKKD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GCNT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human lymph node lysates (calculated MW of 50.9 kDa band according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  GCNT3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GCNT3 Antibody

• beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3
• beta1,6GlcNAc-transferase
• beta1,6-N-acetylglucosaminyltransferase-M
• C2/4GnTGNTM
• C24GNT
• C2GnT2
• C2GNTM
• C2GnT-M
• C2GnT-mucin type
• Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
• EC 2.4.1.102
• EC 2.4.1.150
• glucosaminyl (N-acetyl) transferase 3, mucin type
• hC2GnT-M
• mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

Background

GCNT3 is a glycosyltransferase, belonging to the glycosyltransferase 14 family. It can synthesize all known mucin beta 6 N-acetylglucosaminides and mediates core 2 and core 4 O-glycan branching, two important steps in mucin-type biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCNT3 Antibody (NB100-93400)(1)

Publication using NB100-93400

• Schwientek T, Yeh JC, Levery SB et al. Control of O-glycan branch formation. Molecular cloning and characterization of a novel thymus-associated core 2 beta1, 6-n-acetylglucosaminyltransferase. J Biol Chem 2000-04-14 [PMID: 10753916]

Bioinformatics

• Gene Symbol: GCNT3
• Entrez:
  • 9245(Human)
• Uniprot:
  • NP_004742.1(Human)