GCNF/NR6A1 Antibody

Immunohistochemistry-Paraffin: GCNF/NR6A1 Antibody [NLS5738] - Analysis of anti-NR6A1 / GCNF antibody with human testis.

Product Discontinued

GCNF/NR6A1 Antibody Summary

• Immunogen: Synthetic 18 amino acid peptide from ligand-binding domain of human NR6A1 / GCNF.
• Localization: Nuclear
• Specificity: Human NR6A1 / GCNF. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Zebrafish (94%), Fish (94%), Turkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR6A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 ug/ml

Reactivity Notes

Chimpanzee, Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Elephant (100%), Panda (100%), Platypus (100%), Stickleback (94%), Pufferfish (94%), Xenopus (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GCNF/NR6A1 Antibody

• GCNF
• GCNF1
• GCNFNR61
• Germ cell nuclear factor
• hGCNF
• NR6A1
• nuclear receptor subfamily 6 group A member 1
• nuclear receptor subfamily 6, group A, member 1
• retinoic acid receptor-related testis-associated receptor
• Retinoid receptor-related testis-specific receptor
• RTR
• RTRhRTR

Background

Germ cell nuclear factor (GCNF), a NR6 Germ Cell Nuclear Factor-Like Receptor, has been shown to affect gene expression in embryogenesis and spermatogenesis and is essential for embryonic survival and normal development. Loss of GCNF causes embryonic lethality and disrupts normal somitogenesis as well as neural-tube and axis formation. GCNF binds as a homodimer to the DR0 DNA response element and has been shown to regulate transcription of genes including protamines1 and 2 and Oct4. GCNF's repression function has been shown to be mediated by interaction with the co-repressors N-COR and SMRT in the absence of ligand. Three protein isoforms, GCNF-1, GCNF-2a, and GCNF-2b, have been documented in humans, and they are generated by differential usage of alternative splice acceptor sites of the fourth and seventh exons. GCNF-1, the longest isoform, has a distinct 5 UTR and contains 4 and 26 additional amino acids than the other two forms, respectively. GCNF expression has been documented by in human testis, ovary, prostate, small intesine, and colon. Little or no expression was identified in brain, heart, liver, lung, pancreas, placenta, skeletal muscle, spleen, or thymus. ESTs have been isolated from human tissue libraries, including cancerous cervix, colon, placenta, and nerve in addition to normal testis and embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR6A1
• Entrez:
  • 2649(Human)