GCN2 Overexpression Lysate (Native)

Images

 
Western Blot: GCN2 Lysate [NBL1-10178] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for EIF2AK4

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GCN2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC212459 and based on accession number NM_001013703. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.
Gene
EIF2AK4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
186.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GCN2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GCN2 Overexpression Lysate (Native)

  • EIF2AK4
  • eukaryotic translation initiation factor 2 alpha kinase 4
  • eukaryotic translation initiation factor 2-alpha kinase 4
  • GCN2
  • GCN2GCN2 eIF2alpha kinase
  • GCN2-like protein
  • KIAA1338EC 2.7.11.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GCN2 Lysate (NBL1-10178) (0)

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Video Protocols

WB Video Protocol

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eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK4
Entrez