GCN2 Antibody

Images

 
Western Blot: GCN2 Antibody [NBP1-31679] - Sample (30 ug of whole cell lysate) A: H1299 B: Molt-4 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GCN2 Antibody [NBP1-31679] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: EIF2AK4 protein stained by GCN2 antibody, C-term diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: GCN2 Antibody [NBP1-31679] - Paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.7 mg/ml

Order Details

GCN2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GCN2. The exact sequence is proprietary.
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2AK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
187 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GCN2 Antibody

  • EIF2AK4
  • eukaryotic translation initiation factor 2 alpha kinase 4
  • eukaryotic translation initiation factor 2-alpha kinase 4
  • GCN2
  • GCN2GCN2 eIF2alpha kinase
  • GCN2-like protein
  • KIAA1338EC 2.7.11.1

Background

EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1; MIM 603907) to downregulate protein synthesis in response to varied cellular stresses (Berlanga et al., 1999 [PubMed 10504407]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GCN2 Antibody (NBP1-31679)

Discover related pathways, diseases and genes to GCN2 Antibody (NBP1-31679). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for GCN2 Antibody (NBP1-31679)

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Blogs on GCN2.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK4
Entrez