GCN2 Antibody (JA03-83)

Images

 
Immunocytochemistry/ Immunofluorescence: GCN2 Antibody (JA03-83) [NBP2-66865] - Staining GCN2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: GCN2 Antibody (JA03-83) [NBP2-66865] - Analysis of paraffin-embedded human placenta tissue using anti-GCN2 antibody. Counter stained with hematoxylin.
Flow Cytometry: GCN2 Antibody (JA03-83) [NBP2-66865] - Analysis of MCF-7 cells with GCN2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunocytochemistry/ Immunofluorescence: GCN2 Antibody (JA03-83) [NBP2-66865] - Staining GCN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: GCN2 Antibody (JA03-83) [NBP2-66865] - Staining GCN2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: GCN2 Antibody (JA03-83) [NBP2-66865] - Analysis of paraffin-embedded human breast cancer tissue using anti-GCN2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: GCN2 Antibody (JA03-83) [NBP2-66865] - Analysis of paraffin-embedded human tonsil tissue using anti-GCN2 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JA03-83
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GCN2 Antibody (JA03-83) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Human GCN2 aa 1401-1500
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
EIF2AK4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for GCN2 Antibody (JA03-83)

  • EIF2AK4
  • eukaryotic translation initiation factor 2 alpha kinase 4
  • eukaryotic translation initiation factor 2-alpha kinase 4
  • GCN2
  • GCN2GCN2 eIF2alpha kinase
  • GCN2-like protein
  • KIAA1338EC 2.7.11.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on GCN2.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK4
Entrez