Recombinant Human GBF1 GST (N-Term) Protein

SDS-Page: Recombinant Human GBF1 Protein [H00008729-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human GBF1 GST (N-Term) Protein Summary

• Description: A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 101-209 of Human GBF1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: THEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGT

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Partial Recombinant Protein
• Gene: GBF1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
• Theoretical MW: 37.73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human GBF1 GST (N-Term) Protein

• ARF1GEF
• BFA-resistant GEF 1
• FLJ21263
• GBF1
• golgi brefeldin A resistant guanine nucleotide exchange factor 1
• golgi-specific brefeldin A resistance factor 1
• Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
• KIAA0248
• KIAA0248FLJ21500
• MGC134877
• MGC134878

Background

GBF1 is a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP for GTP on ADP ribosylation factor 5(ARF5). GBF1 activity is inhibited by brefeldin, an antibiotic that interferes with protein transport. It has been show to be important to golgi assembly and the coordination of bidirectional transport. Due to these characteristics, GBF1 is also called Golgi-specific brefeldin A-resistance GEF 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GBF1