GBAS Antibody (OTI1B8) - Azide and BSA Free

Western Blot: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBAS.

Immunocytochemistry/Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GBAS.

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Western Blot: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of extracts (35ug) from 9 different cell lines by using g GBAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of HeLa.

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: GBAS Antibody (OTI1B8) - Azide and BSA Free [NBP2-70804] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI1B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GBAS Antibody (OTI1B8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GBAS Antibody (OTI1B8) - Azide and BSA Free (NBP2-45730) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human GBAS(NP_001474) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NIPSNAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:2000
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GBAS Antibody (OTI1B8) - Azide and BSA Free

• glioblastoma amplified sequence
• Glioblastoma-amplified sequence
• NIPSNAP2NipSnap2,4-nitrophenylphosphatase domain and non-neuronal SNAP25-like 2
• protein NipSnap homolog 2

Background

Chromosomal region 7p12, which contains GBAS, is amplified in approximately 40% of glioblastomas, the most common andmalignant form of central nervous system tumor.The predicted 286-amino acid protein contains a signal peptide, atransmembrane domain, and 2 tyrosine phosphorylation sites. The GBAS transcript is expressed most abundantly in heartand skeletal muscle. GBAS protein might be involved in vesicular transport. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NIPSNAP2