GBAS Antibody (OTI1B8)

Western Blot: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBAS.

Immunohistochemistry: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of extracts (35ug) from 9 different cell lines by usin g GBAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western Blot: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Immunohistochemistry: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescence: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GBAS.

Immunofluorescence: GBAS Antibody (OTI1B8) [NBP2-45730] - Analysis of HeLa.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI1B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GBAS Antibody (OTI1B8) Summary

• Immunogen: Full length human recombinant protein of human GBAS(NP_001474) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NIPSNAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:2000
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GBAS Antibody (OTI1B8)

• glioblastoma amplified sequence
• Glioblastoma-amplified sequence
• NIPSNAP2NipSnap2,4-nitrophenylphosphatase domain and non-neuronal SNAP25-like 2
• protein NipSnap homolog 2

Background

Chromosomal region 7p12, which contains GBAS, is amplified in approximately 40% of glioblastomas, the most common andmalignant form of central nervous system tumor.The predicted 286-amino acid protein contains a signal peptide, atransmembrane domain, and 2 tyrosine phosphorylation sites. The GBAS transcript is expressed most abundantly in heartand skeletal muscle. GBAS protein might be involved in vesicular transport. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GBAS Antibody (NBP2-45730)(1)

Publication using NBP2-45730

• Saha B, Salemi M, Williams GL et al. Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5 alpha in mitophagy Cell reports 2022-05-10 [PMID: 35545034]

Bioinformatics

• Gene Symbol: NIPSNAP2