GATD3A Overexpression Lysate

Images

 
Western Blot: C21orf33 Overexpression Lysate (Adult Normal) [NBL1-08385] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C21orf33.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GATD3A Overexpression Lysate Summary

Description
Transient overexpression lysate of chromosome 21 open reading frame 33 (C21orf33), nuclear gene encoding mitochondrial protein, transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC223892 and based on accession number NM_198155. The protein contains a C-MYC/DDK Tag.
Gene
C21ORF33

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
20.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GATD3A Overexpression Lysate

  • chromosome 21 open reading frame 33
  • D21S2048E
  • ES1
  • GT335
  • HES1
  • HES1KNP-Ia
  • human HES1 protein, homolog to E.coli and zebrafish ES1 protein, 10Protein GT335
  • Keio novel protein I
  • KNPH
  • KNPI
  • KNP-I
  • KNPImitochondrial
  • Protein KNP-I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-78486
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NBP2-41305
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF2567
Species: Mu
Applications: IHC, WB
NBP1-33427
Species: Hu
Applications: ChIP, ICC/IF, WB
NB100-93551
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF1190
Species: Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
NBP2-16818
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, WB
NBP1-84257
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-83957
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
H00004854-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF3444
Species: Hu
Applications: IHC, WB
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP3-03905
Species: Hu, Mu, Rt
Applications: WB
1845-SH/CF
Species: Hu
Applications: BA
NBP1-45693
Species: Hu, Mu, Rt
Applications: IHC, WB
5026-DL
Species: Mu
Applications: BA
NBP1-89090
Species: Hu
Applications: IHC, IHC-P

Publications for GATD3A Lysate (NBL1-08385) (0)

There are no publications for GATD3A Lysate (NBL1-08385).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GATD3A Lysate (NBL1-08385) (0)

There are no reviews for GATD3A Lysate (NBL1-08385). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GATD3A Lysate (NBL1-08385) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GATD3A Products

Bioinformatics Tool for GATD3A Lysate (NBL1-08385)

Discover related pathways, diseases and genes to GATD3A Lysate (NBL1-08385). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GATD3A Lysate (NBL1-08385)

Discover more about diseases related to GATD3A Lysate (NBL1-08385).
 

Pathways for GATD3A Lysate (NBL1-08385)

View related products by pathway.

PTMs for GATD3A Lysate (NBL1-08385)

Learn more about PTMs related to GATD3A Lysate (NBL1-08385).

Blogs on GATD3A

There are no specific blogs for GATD3A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GFAP Antibody
NB300-141

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GATD3A Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol C21ORF33