gamma Tubulin Antibody (TU-32)

Images

 
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y ...read more
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.

Product Details

Summary
Reactivity Po, An, Pl, PrSpecies Glossary
Applications WB, ICC/IF
Clone
TU-32
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

gamma Tubulin Antibody (TU-32) Summary

Immunogen
human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
Epitope
The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Marker
Centrosome Marker
Specificity
The TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBG1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Immunocytochemistry: methanol/acetone fixation required

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for gamma Tubulin Antibody (TU-32)

  • gamma-1-tubulin
  • Gamma-tubulin complex component 1
  • GCP-1
  • TUBG
  • TUBGCP1
  • tubulin gamma-1 chain
  • tubulin, gamma 1
  • tubulin, gamma polypeptide

Background

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma Tubulin Antibody (NB110-90616) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-90616AF405
Alexa Fluor 488 Labeled NB110-90616AF488
Alexa Fluor 647 Labeled NB110-90616AF647
Alexa Fluor 700 Labeled NB110-90616AF700
DyLight 350 Labeled NB110-90616UV
DyLight 405 Labeled NB110-90616V
DyLight 488 Labeled NB110-90616G
DyLight 680 Labeled NB110-90616FR
DyLight 755 Labeled NB110-90616IR

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Bioinformatics

Gene Symbol TUBG1
Entrez
Uniprot