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gamma Tubulin Antibody (TU-30) - BSA Free

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Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell ...read more
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of HeLa cells using anti-gamma Tubulin antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer ...read more
Immunohistochemistry-Paraffin: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574] - FFPE Glioblastoma PDX is stained with mouse-gamma tubulin antibody (NB500-574) (red) for centrosomes and rabbit Arl13B (green) for ...read more
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat ...read more
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI ...read more
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained ...read more
Analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Pl, PrSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
TU-30
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

gamma Tubulin Antibody (TU-30) - BSA Free Summary

Immunogen
C-terminal peptide of gamma-tubulin counjugated to KLH.
Epitope
The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.
Marker
Centrosome Marker
Specificity
The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBG1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
Application Notes
Immunocytochemistry - Staining technique: (a) Fix cells for 10 min in methanol at -20oC and for 6 min in acetone at -20oC; (b) Fix cells directly in methanol for 10 min at -20oC or in acetone for 10 min at -20oC. Incubation: 45 min in room temperature Application note: The antibody TU-30 stains only fixed cells. gamma Tubulin Antibody (TU-30) - BSA Free validated for Immunohistochemistry-Paraffin from a verified customer review.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB500-574 in the following applications:

Publications
Read Publication using
NB500-574 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for gamma Tubulin Antibody (TU-30) - BSA Free

  • gamma Tubulin
  • Gamma-1-tubulin
  • Gamma-tubulin complex component 1
  • GCP-1
  • TUBG
  • TUBG1
  • TUBGCP1
  • tubulin gamma-1 chain
  • tubulin, gamma 1
  • tubulin, gamma polypeptide

Background

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-574
Species: Hu, Mu, Rt, Po, Bv, Ch, Pl, Pr
Applications: WB, Flow, ICC/IF, IHC

Publications for gamma Tubulin Antibody (NB500-574)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Human
(1)
Mouse
(1)
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Reviews for gamma Tubulin Antibody (NB500-574) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB500-574:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin gamma Tubulin NB500-574
Enlarge
4
reviewed by:
Verified Customer
IHC-P Human 09/06/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman glioblastoma
SpeciesHuman
Lot538514

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Immunofluorescence gamma Tubulin NB500-574
Enlarge
5
reviewed by:
Verified Customer
IF Human 08/16/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
Lot524972

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gamma Tubulin Antibody (NB500-574) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
09/06/2022
Application: IHC-P
Species: Human

Verified Customer
08/16/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol TUBG1