gamma-Synuclein Antibody [Unconjugated] (AF5745)

Western blot shows lysates of human brain tissue. PVDF Membrane was probed with 1 µg/mL of Human gamma -Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5745) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma -Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

gamma -Synuclein was detected in immersion fixed paraffin-embedded sections of human cerebellum using Human gamma -Synuclein Antigen Affinity Purified Polyclonal Antibody (Catalog # AF5745) at 1.7 µg/mL overnight at4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

gamma-Synuclein Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human gamma -Synuclein
  Asn47-Glu120
  Accession # O76070
• Specificity: Detects human gamma -Synuclein in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human
  beta -Synuclein is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SNCG

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gamma-Synuclein Antibody [Unconjugated]

• BCSG1
• BCSG1PERSYN
• Breast cancer-specific gene 1 protein
• gamma-Synuclein
• persyn
• PRSN
• SNCG
• SRPRSN
• Synoretin
• synuclein, gamma (breast cancer-specific protein 1)
• Synuclein-gamma

Background

gamma -Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNCG
• Entrez:
  • 6623(Human)
• Uniprot:
  • O76070()