HAF007). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human hypothalamus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5745-SP | |||
HAF016). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9." alt="Western blot shows lysates of human brain tissue. PVDF Membrane was probed with 1 µg/mL of Human gamma-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5745) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5745-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60497-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20099-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-Immunocytochemistry-NBP1-31209-img0006.jpg "Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: gamma-Synuclein Antibody [NBP1-31209] - Mouse fore brain. gamma Synuclein antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-31209-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Wild-type (WT) and gamma Synuclein knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma-Synuclein antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/gamma-Synuclein-Antibody-Western-Blot-NBP1-31209-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma Synuclein antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31209_rabbit-polyclonal-gamma-synuclein-antibody-1610202419405744.jpg)
CTS019) and counterstained with hematoxylin (blue). View our protocol for ![Western Blot: gamma-Synuclein Antibody (1H10D2) [NB110-60497] - Analysis using mouse mAb against truncated SNCG recombinant protein.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-1H10D2-Western-Blot-NB110-60497-img0002.jpg "Western Blot: gamma-Synuclein Antibody (1H10D2) [NB110-60497] - Analysis using mouse mAb against truncated SNCG recombinant protein.")
![Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (1H10D2) [NB110-60497] - Analysis of human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic(ovary carcinoma) localization, cytoplasmic and nuclear (breast carcinoma) localization using SNCG mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Synuclein-gamma-Antibody-1H10D2-Immunohistochemistry-Paraffin-NB110-60497-img0004.jpg)
![Western Blot: gamma-Synuclein Antibody (4A0F4) [NBP3-16144] - Western blot analysis of extracts of various cell lines, using gamma-Synuclein Rabbit mAb (NBP3-16144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/gamma-Synuclein-Antibody-4A0F4-Western-Blot-NBP3-16144-img0002.jpg "Western Blot: gamma-Synuclein Antibody (4A0F4) [NBP3-16144] - Western blot analysis of extracts of various cell lines, using gamma-Synuclein Rabbit mAb (NBP3-16144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (4A0F4) [NBP3-16144] - Immunohistochemistry of paraffin-embedded rat brain using gamma-Synuclein Rabbit mAb (NBP3-16144) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/gamma-Synuclein-Antibody-4A0F4-Immunohistochemistry-Paraffin-NBP3-16144-img0001.jpg)
![In vitro assay: Recombinant Human gamma-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP3-14780] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in gamma synuclein fibrils. Increased fluorescence (correlated to gamma synuclein fibrillization) is seen when human gamma synuclein monomer is combined with human gamma synuclein PFFs (NBP3-14780).](http://images.novusbio.com/fullsize/Recombinant-Human-gamma-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP3-14780-img0001.jpg "In vitro assay: Recombinant Human gamma-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP3-14780] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in gamma synuclein fibrils. Increased fluorescence (correlated to gamma synuclein fibrillization) is seen when human gamma synuclein monomer is combined with human gamma synuclein PFFs (NBP3-14780).")
![Electron Microscopy: Recombinant Human gamma-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP3-14780] - TEM of Active Human Recombinant gamma-Synuclein Protein Pre-formed Fibrils (Type 1) (NBP3-14780)](http://images.novusbio.com/fullsize/Recombinant-Human-gamma-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP3-14780-img0002.jpg)
![Flow Cytometry: gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free [NBP3-20099] - Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (NBP3-20099, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20099_rabbit-gamma-synuclein-mab-saic-31a-10-azide-bsa-free-2742023924224.png "Flow Cytometry: gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free [NBP3-20099] - Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (NBP3-20099, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (SAIC-31A-10) - Azide and BSA Free [NBP3-20099] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-31A-10 (NBP3-20099) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20099, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20099_rabbit-gamma-synuclein-mab-saic-31a-10-azide-bsa-free-2742023924228.png)
![SDS-Page: Recombinant Human gamma-Synuclein Protein [NBC1-18329] - gamma-Synuclein, 13.3 kDa (127 aa) , confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/gamma-Synuclein-Protein-SDS-Page-NBC1-18329-img0002.jpg "SDS-Page: Recombinant Human gamma-Synuclein Protein [NBC1-18329] - gamma-Synuclein, 13.3 kDa (127 aa) , confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![In vitro assay: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in gamma synuclein fibrils. Increased fluorescence (correlated to gamma synuclein fibrillization) is seen when human gamma synuclein monomer (NBP3-14778) is combined with human gamma synuclein PFFs .](http://images.novusbio.com/fullsize/Recombinant-Human-gamma-Synuclein-Active--Monomer-Protein-In-vitro-assay-NBP3-14778-img0001.jpg "In vitro assay: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in gamma synuclein fibrils. Increased fluorescence (correlated to gamma synuclein fibrillization) is seen when human gamma synuclein monomer (NBP3-14778) is combined with human gamma synuclein PFFs .")
![SDS-Page: Recombinant Human gamma-Synuclein Active, Monomer Protein [NBP3-14778] - Coomassie gel stain of Human gamma-Synuclein Monomer (NBP3-14778). Load: 2 ug.](http://images.novusbio.com/fullsize/Recombinant Human gamma-Synuclein Active- Monomer Protein-SDS-Page-NBP3-14778-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-89996_rabbit-polyclonal-gamma-synuclein-antibody-15520258544735.jpg)
![ELISA: Human gamma-Synuclein ELISA Kit (Colorimetric) [NBP3-08176] - Samples were spiked with high concentrations of Human gamma-Synuclein and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08176_human-gamma-synuclein-elisa-kit-colorimetric-13220241685321.jpg "ELISA: Human gamma-Synuclein ELISA Kit (Colorimetric) [NBP3-08176] - Samples were spiked with high concentrations of Human gamma-Synuclein and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human gamma-Synuclein ELISA Kit (Colorimetric) [NBP3-08176] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08176_human-gamma-synuclein-elisa-kit-colorimetric-4320249173415.jpg)
![SDS-Page: Recombinant Mouse gamma-Synuclein Protein [NBP2-61179] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Enolase-1-Protein-SDS-Page-NBP2-61179-img0001.jpg "SDS-Page: Recombinant Mouse gamma-Synuclein Protein [NBP2-61179] - 15 % SDS-PAGE (3ug)")
