Human Galectin-8 ELISA Kit (Colorimetric)

ELISA: Human Galectin-8 ELISA Kit (Colorimetric) [NBP3-00386] - Samples were spiked with high concentrations of Human Galectin-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Galectin-8 ELISA Kit (Colorimetric) [NBP3-00386] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Galectin-8 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Galectin-8 in samples. No significant cross-reactivity or interference between Human Galectin-8 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.88%
• Intra-Assay: 5.61%
• Spike Recovery: 85 - 102%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LGALS8

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Galectin-8 ELISA Kit (Colorimetric)

• GAL8
• Gal-8
• galectin 8
• Galectin8
• Galectin-8
• galectin-8g
• lectin, galactoside-binding, soluble, 8
• LGALS8
• PCTA1
• PCTA-1Po66 carbohydrate-binding protein
• Po66 carbohydrate binding protein
• Po66-CBP
• Prostate carcinoma tumor antigen 1

Background

Several proteins have been identified as specific markers of prostate cancer, and they may be useful as diagnostic indicators. PSA, prostate specific antigen, is the classical indicator for transformed prostate tissue; however, in addition to being upregulated in prostate cancer, PSA is also upregulated in non-malignant conditions, such as benign prostatic hyperplasia prostate. Galectin-8, also known as prostate-specific membrane antigen (PCTA-1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates both cell-cell and cellmatrix interactions in a manner similar to the selectin subgroup of C-type lectins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Galectin-8 ELISA Kit (NBP3-00386). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LGALS8