GADD45 alpha Overexpression Lysate (Native)

Images

 
Western Blot: GADD45A Overexpression Lysate (Adult Normal) [NBL1-10934] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GADD45A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GADD45 alpha Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204005 and based on accession number NM_001924. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens growth arrest and DNA-damage-inducible, alpha (GADD45A), mRNA.
Gene
GADD45A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GADD45 alpha

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GADD45 alpha Overexpression Lysate (Native)

  • DDIT-1
  • DDIT1growth arrest and DNA damage-inducible protein GADD45 alpha
  • DNA damage-inducible transcript 1 protein
  • DNA damage-inducible transcript-1
  • GADD45 alpha
  • GADD45a
  • GADD45DNA-damage-inducible transcript 1
  • growth arrest and DNA-damage-inducible 45 alpha
  • growth arrest and DNA-damage-inducible, alpha

Background

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GADD45 alpha Lysate (NBL1-10934) (0)

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Bioinformatics

Gene Symbol GADD45A
Entrez