GADD34 Antibody (OTI3D12)

Images

 
Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).
Immunocytochemistry/ Immunofluorescence: GADD34 Antibody (3D12) [NBP2-01787] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.
Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.
Flow Cytometry: GADD34 Antibody (3D12) [NBP2-01787] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).
Western Blot: GADD34 Antibody (3D12) [NBP2-01787] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Western Blot: GADD34 Antibody (3D12) [NBP2-01787] Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI3D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.25 mg/ml

Order Details

GADD34 Antibody (OTI3D12) Summary

Immunogen
Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
Marker
ER Stress Marker
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PPP1R15A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
73.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
GADD34 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP2-01787 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.25 mg/ml
Purity
Affinity purified

Alternate Names for GADD34 Antibody (OTI3D12)

  • GADD34Growth arrest and DNA damage-inducible protein GADD34
  • growth arrest and DNA-damage-inducible 34
  • Myeloid differentiation primary response protein MyD116 homolog
  • protein phosphatase 1 regulatory subunit 15A
  • protein phosphatase 1, regulatory (inhibitor) subunit 15A

Background

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GADD34 Antibody (NBP2-01787)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD34 Antibody (NBP2-01787) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71765AF350
Alexa Fluor 405 NBP2-71765AF405
Alexa Fluor 488 NBP2-71765AF488
Alexa Fluor 532 NBP2-71765AF532
Alexa Fluor 594 NBP2-71765AF594
Alexa Fluor 647 NBP2-71765AF647
Alexa Fluor 700 NBP2-71765AF700
Alexa Fluor 750 NBP2-71765AF750
Allophycocyanin NBP2-71765APC
Biotin NBP2-71765B
DyLight 350 NBP2-71765UV
DyLight 405 NBP2-71765V
DyLight 488 NBP2-71765G
DyLight 550 NBP2-71765R
DyLight 594 NBP2-71765DL594
DyLight 650 NBP2-71765C
DyLight 680 NBP2-71765FR
DyLight 755 NBP2-71765IR
FITC NBP2-71765F
HRP NBP2-71765H
Janelia Fluor 549 NBP2-71765JF549
Janelia Fluor 646 NBP2-71765JF646
PE NBP2-71765PE
PerCP NBP2-71765PCP

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Bioinformatics

Gene Symbol PPP1R15A
Entrez