GAB3 Antibody (720306)

Western blot shows lysates of human spleen tissue and human thymus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GAB3 Monoclonal Antibody (Catalog # MAB7127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAB3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 720306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GAB3 Antibody (720306) Summary

• Immunogen: E. coli-derived recombinant human GAB3
  Leu121-Asn263
  Accession # Q8WWW8
• Specificity: Detects human GAB-3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAB3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAB3 Antibody (720306)

• DOS/Gab family member 3
• Gab3 scaffolding protein
• GAB3
• GRB2-associated binder 3
• GRB2-associated binding protein 3
• GRB2-associated-binding protein 3
• Growth factor receptor bound protein 2-associated protein 3

Background

GAB3 (GRB2-associated-binding protein 3) is a ~75-80 kDa member of the Gab family of scaffolding proteins which show overlapping expression in hematopoietic cells. All contain an N-terminal pleckstrin homology domain and multiple tyrosine phosphorylation and SH2 and SH3 domain binding sites.  M-CSF stimulation of myeloid precursors  through M-CSF R co-induces GAB3 and the associated adaptor protein GRAP2, and causes macrophage differentiation associated with tyrosine phosphorylation of GAB3. Over amino acids (aa) 121-263, human GAB3 shares 73% aa identity with mouse GAB3.  A potential isoform of 548 aa lacks aa 509-548, while another of 488 aa lacks aa 173-356 and diverges at aa 475. A third potential isoform of 206 aa has an alternate start site at aa 380.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAB3
• Uniprot:
  • Q8WWW8()