G protein alpha Inhibitor 2 Antibody (3G11) - Azide and BSA Free

Western Blot: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02] - GNAI2 monoclonal antibody (M02), clone 3G11. Analysis of GNAI2 expression in MCF-7.

Proximity Ligation Assay: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02] - Analysis of protein-protein interactions between C5AR1 and GNAI2. HeLa cells were stained with anti-C5AR1 rabbit purified polyclonal 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: G protein alpha Inhibitor 2 Antibody (3G11) [H00002771-M02] - Detection limit for recombinant GST tagged GNAI2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func
• Clone: 3G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

G protein alpha Inhibitor 2 Antibody (3G11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GNAI2 (AAH12138, 1 a.a. ~ 355 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
• Specificity: GNAI2 - guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GNAI2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for G protein alpha Inhibitor 2 Antibody (3G11) - Azide and BSA Free

• alpha-2 subunit
• guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 2

Background

RBM5 (RNA binding motif protein 5) is a nuclear SR-related RNA binding protein which specifically binds poly(G) RNA homopolymers in vitro. It modulates both apoptosis and the cell cycle, and retards tumor formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNAI2
• Entrez:
  • 2771(Human)
• OMIM:
  • 139360(Human)
• Uniprot:
  • AAH12138(Human)