FXR2 Antibody (1G2) - BSA Free

Western Blot: FXR2 Antibody (1G2) [NB300-259] - Whole cell HeLa lysate at a concentration of 1:2500 (lane 1) and 1:500 (lane 2).

Immunocytochemistry/Immunofluorescence: FXR2 Antibody (1G2) [NB300-259] - FXR2 (1G2) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

FXR2 Antibody (1G2) - BSA Free Summary

• Immunogen: Recombinant C-terminal fragment of human FXR2 (residues 414-658). [UniProt# P51116]
• Localization: Cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FXR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Western Blot 1:500-1:2500
• Application Notes: This FXR2 Antibody (1G2) is useful for Immunohistochemistry on paraffin-embedded secitons and Western blot, where a band is seen at ~80 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for FXR2 Antibody (1G2) - BSA Free

• FMR1L2
• fragile X mental retardation syndrome-related protein 2
• fragile X mental retardation, autosomal homolog 2
• fragile X-mental retardation 1-like 2
• FXR2P

Background

FXR2 (Fragile X relative protein 2) belongs to FMR1 family/fragile X mental retardation proteins which can bind to RNA and associate with polyribosomes. All three members of FMR1 proteins i.e. FMRP, FXR1, and FXR2 contains RNA-binding domains and RGG boxes, and are found predominantly in cytoplasm where they can associate with ribosomes for implicating translation or mRNA stability. FXR2 has been shown to interact with FMR1, FXR1, CYFIP2 and TDRD3. It is ubiquitously expressed during embryonic development and its tissue distribution overlaps with FMRP in adult stages. However, interestingly, FXR2 knockout mice display learning impairments and changes in synaptic plasticity that are somewhat distinct from those of FMRP-deficient mice, indicating that FXR2 may also regulate adult hippocampal neurogenesis via mechanisms distinct from FMRP. FXR2 deficiency leads to altered stem cell proliferation and differentiation specifically in dentate gyrus and not in subventricular zone which has been suggested as a key component of mechanism regulating differential neurogenic processes in these two adult germinal zones.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FXR2 Antibody (NB300-259)(2)

Publications using NB300-259

• Gao Q, Bi P, Mi Q et al. Effect of nicotine on cholesterol gallstone formation in C57BL/6J mice fed on a lithogenic diet Experimental and therapeutic medicine 2023-02-01 [PMID: 36684657] (IHC-P, WB, Mouse)
  
  Details:
  Dilution used in IHC-P 1:500, WB 1:2,000
• Darnell JC, Fraser CE, Mostovetsky O, Darnell RB. Discrimination of common and unique RNA-binding activities among Fragile X mental retardation protein paralogs. Hum Mol Genet;18(17):3164-77. 2009-09-01 [PMID: 19487368] (WB, Mouse)

FAQs for FXR2 Antibody (NB300-259). (Showing 1 - 1 of 1 FAQs).

1. Can you please tell me if your FXR antibody is purified from ascites or from tissue culture supernatant? Does your material for purification vary from lot to lot? If so, the information for the current lot will be sufficient.
   • We prefer not to vary the type of production unless necessary. This item is purified from ascites.

Bioinformatics

• Gene Symbol: FXR2