FXR2 Antibody (1G2) - BSA Free

Images

 
Western Blot: FXR2 Antibody (1G2) [NB300-259] - Whole cell HeLa lysate at a concentration of 1:2500 (lane 1) and 1:500 (lane 2).
Immunocytochemistry/ Immunofluorescence: FXR2 Antibody (1G2) [NB300-259] - FXR2 (1G2) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
1G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

FXR2 Antibody (1G2) - BSA Free Summary

Immunogen
Recombinant C-terminal fragment of human FXR2 (residues 414-658). [UniProt# P51116]
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FXR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Western Blot 1:500-1:2500
Application Notes
This FXR2 Antibody (1G2) is useful for Immunohistochemistry on paraffin-embedded secitons and Western blot, where a band is seen at ~80 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publication using
NB300-259 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FXR2 Antibody (1G2) - BSA Free

  • FMR1L2
  • fragile X mental retardation syndrome-related protein 2
  • fragile X mental retardation, autosomal homolog 2
  • fragile X-mental retardation 1-like 2
  • FXR2P

Background

FXR2 (Fragile X relative protein 2) belongs to FMR1 family/fragile X mental retardation proteins which can bind to RNA and associate with polyribosomes. All three members of FMR1 proteins i.e. FMRP, FXR1, and FXR2 contains RNA-binding domains and RGG boxes, and are found predominantly in cytoplasm where they can associate with ribosomes for implicating translation or mRNA stability. FXR2 has been shown to interact with FMR1, FXR1, CYFIP2 and TDRD3. It is ubiquitously expressed during embryonic development and its tissue distribution overlaps with FMRP in adult stages. However, interestingly, FXR2 knockout mice display learning impairments and changes in synaptic plasticity that are somewhat distinct from those of FMRP-deficient mice, indicating that FXR2 may also regulate adult hippocampal neurogenesis via mechanisms distinct from FMRP. FXR2 deficiency leads to altered stem cell proliferation and differentiation specifically in dentate gyrus and not in subventricular zone which has been suggested as a key component of mechanism regulating differential neurogenic processes in these two adult germinal zones.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FXR2 Antibody (NB300-259)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for FXR2 Antibody (NB300-259): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FXR2 Antibody (NB300-259). (Showing 1 - 1 of 1 FAQ).

  1. Can you please tell me if your FXR antibody is purified from ascites or from tissue culture supernatant? Does your material for purification vary from lot to lot? If so, the information for the current lot will be sufficient.
    • We prefer not to vary the type of production unless necessary. This item is purified from ascites.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FXR2