FXR1 Antibody - BSA Free

Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.

Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22246 used for IP at 6 ug/mg lysate. FXR1 was also immunoprecipitated by rabbit anti-FXR1 antibody. For blotting immunoprecipitated FXR1, NBP2-22246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Adult mouse heart. A: 30 ug whole cell lysate. B: IgG-IP. C: Fxr1-antibody-IP. 10% SDS-PAGE, antibody diluted 1:2000 in WB assay and 3ug/mg in IP assay. Image submitted by a verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FXR1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FXR1 Antibody - BSA Free (NBP2-22246) is a polyclonal antibody validated for use in WB and IP. Anti-FXR1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 571 and 621 of human Fragile X Mental Retardation, Autosomal Homolog 1 using the numbering given in entry NP_005078.2 (GeneID 8087).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FXR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FXR1 Antibody - BSA Free

• fragile X mental retardation syndrome-related protein 1
• fragile X mental retardation, autosomal homolog 1
• FXR1P
• hFXR1p

Background

FXR1 is encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FXR1 Antibody (NBP2-22246)(3)

Publications using NBP2-22246

• Chen X, Fansler MM, Janjoš U, Ule J et Al. The FXR1 network acts as a signaling scaffold for actomyosin remodeling Cell 2024-08-06 [PMID: 39106863]
• Chen X, Fansler MM, Janjoš U et al. The FXR1 network acts as signaling scaffold for actomyosin remodeling bioRxiv : the preprint server for biology 2023-11-05 [PMID: 37961296] (IP)
• Kang JY, Wen Z, Pan D et al. LLPS of FXR1 drives spermiogenesis by activating translation of stored mRNAs Science (New York, N.Y.) 2022-08-12 [PMID: 35951695] (WB, Mouse)

Review for FXR1 Antibody (NBP2-22246) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IP Mouse 04/06/2018

Summary

• Application: Immunoprecipitation
• Sample Tested: Adult heart
• Species: Mouse
• Lot: A

Bioinformatics

• Gene Symbol: FXR1
• Uniprot:
  • NP_005078.2(Human)