FXR1 Antibody (JE40-57)

Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunocytochemistry analysis of HeLa cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: FXR1 Antibody (JE40-57) [NBP3-32354] - Flow cytometric analysis of HeLa cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32354, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JE40-57
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FXR1 Antibody (JE40-57) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human FXR1 aa 21-70 / 621. (Uniprot: P51114)
• Localization: Cytoplasm, cytosol.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FXR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FXR1 Antibody (JE40-57)

• fragile X mental retardation syndrome-related protein 1
• fragile X mental retardation, autosomal homolog 1
• FXR1P
• hFXR1p

Background

FXR1 is encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FXR1