FUSIP1 Antibody

Western Blot: FUSIP1 Antibody [NB110-93598] - Detection of human FUSIP1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-FUSIP1 antibody NB110-93598 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunocytochemistry/Immunofluorescence: FUSIP1 Antibody [NB110-93598] - FUSIP1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: FUSIP1 Antibody [NB110-93598] - Detection of human FUSIP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FUSIP1 (NB110-93598) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: FUSIP1 Antibody [NB110-93598] - Detection of FUSIP1, Isoform 3, in HepG2 whole cell lysates using NB110-93598.

Product Discontinued

FUSIP1 Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of human FUSIP1 (within residues 100-200). [Swiss-Prot# O75494]
• Localization: Nucleus speckle. Cytoplasm.
• Specificity: This is specific for isoform 3 of the FUSIP1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRSF10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 -1:5000
• Application Notes: This FUSIP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~22 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FUSIP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FUSIP1 Antibody

• 40 kDa SR-repressor protein
• FLJ43846
• FUS interacting protein (serine-arginine rich) 1
• FUS-interacting protein (serine-arginine rich) 2
• FUS-interacting serine-arginine-rich protein 1
• FUSIP1DKFZp686H0644
• FUSIP2
• neural-salient SR protein
• NSSR
• serine/arginine-rich splicing factor 10
• serine-arginine repressor protein (40 kDa)
• SFRS13
• SFRS13A
• Splicing factor SRp38
• splicing factor, arginine/serine-rich 13
• Splicing factor, arginine/serine-rich 13ATLS-associated protein with Ser-Arg repeats
• SR splicing factor 10
• SRp38
• SRrp40TLS-associated protein with SR repeats
• TASR1TLS-associated SR protein
• TASR2FUS interacting protein (serine/arginine-rich) 1
• TASRFLJ30749
• TLS-associated protein TASR
• TLS-associated serine-arginine protein 1
• TLS-associated serine-arginine protein 2
• TLS-associated serine-arginine protein

Background

FUSIP1 is a member of the serine-arginine (SR) family of proteins, which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. FUSIP1 can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and abrogates the influence of TLS on E1A pre-mRNA splicing. Alternative splicing of this gene results in at least two transcript variants encoding different isoforms. This antibody is specific for isoform 3 of FUSIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FUSIP1 Antibody (NB110-93598)(2)

Publications using NB110-93598

• Fan Y, Zhang Y, Zhao H et al. lncR-GAS5 upregulates the splicing factor SRSF10 to impair endothelial autophagy, leading to atherogenesis Frontiers of medicine 2023-04-01 [PMID: 36645633]
• Liu W, Lu X, Zhao ZH et al. SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing eLife 2022-11-10 [PMID: 36355419] (WB, Mouse)

Bioinformatics

• Gene Symbol: SRSF10
• Entrez:
  • 10772(Human)
  • 14105(Mouse)
• Uniprot:
  • O75494(Human)