FUSIP1 Overexpression Lysate

Western Blot: FUSIP1 Overexpression Lysate (Adult Normal) [NBL1-10862] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FUSIP1.

• Reactivity: Hu
• Applications: WB

FUSIP1 Overexpression Lysate Summary

• Description:

  FUSIP1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC221759
  
  Accession#: NM_006625
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: SRSF10

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for FUSIP1 Overexpression Lysate

• 40 kDa SR-repressor protein
• FLJ43846
• FUS interacting protein (serine-arginine rich) 1
• FUS-interacting protein (serine-arginine rich) 2
• FUS-interacting serine-arginine-rich protein 1
• FUSIP1DKFZp686H0644
• FUSIP2
• neural-salient SR protein
• NSSR
• serine/arginine-rich splicing factor 10
• serine-arginine repressor protein (40 kDa)
• SFRS13
• SFRS13A
• Splicing factor SRp38
• splicing factor, arginine/serine-rich 13
• Splicing factor, arginine/serine-rich 13ATLS-associated protein with Ser-Arg repeats
• SR splicing factor 10
• SRp38
• SRrp40TLS-associated protein with SR repeats
• TASR1TLS-associated SR protein
• TASR2FUS interacting protein (serine/arginine-rich) 1
• TASRFLJ30749
• TLS-associated protein TASR
• TLS-associated serine-arginine protein 1
• TLS-associated serine-arginine protein 2
• TLS-associated serine-arginine protein

Background

This gene product is a member of the serine-arginine (SR) family of proteins, which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and abrogates the influence of TLS on E1A pre-mRNA splicing. Alternative splicing of this gene results in at least two transcript variants encoding different isoforms. In addition, transcript variants utilizing alternative polyA sites exist. Transcript Variant: This variant (1) uses an alternate last exon resulting in a frameshift and an earlier stop codon, as compared to variant 2. Isoform 1 is 79 aa shorter than isoform 2 and has a distinct C-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SRSF10