Fucosyltransferase 7/FUT7 Antibody (795116) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 795116
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Fucosyltransferase 7/FUT7 Antibody (795116) [Alexa Fluor® 405] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 7/FUT7
  Ala36-Ala342
  Accession # Q11130
• Specificity: Detects human Fucosyltransferase 7/FUT7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Fucosyltransferase 5 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fucosyltransferase 7/FUT7 Antibody (795116) [Alexa Fluor® 405]

• alpha-(1,3)-fucosyltransferase
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• FTVII
• fucosyltransferase 7 (alpha (1,3) fucosyltransferase)
• Fucosyltransferase 7
• Fucosyltransferase VII
• Fuc-TVII
• FucT-VII
• FUT7
• Galactoside 3-L-fucosyltransferase
• Selectin ligand synthase
• selectin-ligand synthase

Background

N-glycans, O-glycans and glycolipids are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with important biological function. Well known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in the leukocyte homing and extravasation process and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned (2). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (3). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha 1-3 or alpha 1-4 fucosyltransferases and are responsible for Lewis antigen generation. FUT7 plays an exclusive role for the biosynthesis of sialyl Lewis X epitope (NeuAc alpha 2,3Gal beta 1,4[Fuc alpha 1,3]GlcNAc) that serves as a ligand in the E-selectin and P-selectin mediated adhesion of leukocytes to activated endothelium or platelets (4, 5, 6). Expression of FUT7 is increased by hypoxia in human colon cancer cell lines (7). Within aa 36-342, human FUT7 shares 84% aa sequence identity with mouse and rat FUT7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q11130(Human)