Fucosyltransferase 7/FUT7 Antibody (795116) [Alexa Fluor® 700]

• Applications: WB, ICC/IF
• Clone: 795116
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Fucosyltransferase 7/FUT7 Antibody (795116) [Alexa Fluor® 700] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 7/FUT7
  Ala36-Ala342
  Accession # Q11130
• Specificity: Detects human Fucosyltransferase 7/FUT7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Fucosyltransferase 5 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

N-glycans, O-glycans and glycolipids are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with important biological function. Well known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in the leukocyte homing and extravasation process and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned (2). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (3). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha 1-3 or alpha 1-4 fucosyltransferases and are responsible for Lewis antigen generation. FUT7 plays an exclusive role for the biosynthesis of sialyl Lewis X epitope (NeuAc alpha 2,3Gal beta 1,4[Fuc alpha 1,3]GlcNAc) that serves as a ligand in the E-selectin and P-selectin mediated adhesion of leukocytes to activated endothelium or platelets (4, 5, 6). Expression of FUT7 is increased by hypoxia in human colon cancer cell lines (7). Within aa 36-342, human FUT7 shares 84% aa sequence identity with mouse and rat FUT7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.