FRMPD1 Antibody - BSA Free

Western Blot: FRMPD1 Antibody [NBP1-77082] - K562 cell lysate with FRMPD1 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: FRMPD1 Antibody - BSA Free [NBP1-77082] - Immunofluorescence of FRMPD1 in K562 cells with FRMPD1 antibody at 20 u/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FRMPD1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FRMPD1 Antibody - BSA Free (NBP1-77082) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FRMPD1. The immunogen is located within amino acids 1330 - 1380 of FRMPD1. Amino Acid Squence: CFRGLQPETEEEDED
• Predicted Species: Mouse (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FRMPD1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Western Blot 1-2 ug/ml
• Control Peptide:

  FRMPD1 Peptide (NBP1-77082PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FRMPD1 Antibody - BSA Free

• FERM and PDZ domain containing 1
• FERM and PDZ domain-containing protein 1
• FRMD2KIAA0967FERM domain-containing protein 2
• MGC138833

Background

The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD1 was initially identified through a yeast two-hybrid screening with Activator of G-protein signaling 3 (AGS3), a protein that acts to stabilize the GDP-bound conformation of Gai, as the bait. FRMPD1 binds to the tetratricopeptide repeat of AGS3 and stabilizes AGS3 in a membrane environment. Suppression of FRMPD1 in Cath.a-differentiated neuronal cells decreased the level of endogenous AGS3 in membrane fractions by ~50% and enhanced the inhibition of forskolin-induced increases in cAMP. Co-immunoprecipitation studies indicate that the interaction of AGS3 with FRMPD1 is mutually exclusive with the interaction of AGS3 with Gai3, suggesting that FRMPD1 may position AGS3 in the membrane where it can then interact with Galphai.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FRMPD1
• Uniprot:
  • NP_055722(Human)