Frizzled-7 Antibody (151143) [Unconjugated]

Frizzled-7 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human/Mouse Frizzled-7 Monoclonal Antibody (Catalog # MAB1981) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Product Discontinued

Frizzled-7 Antibody (151143) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
  Gln33-Leu185
  Accession # Q61090
• Specificity: Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FZD7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-7 Antibody (151143) [Unconjugated]

• frizzled (Drosophila) homolog 7
• frizzled homolog 7 (Drosophila)
• Frizzled, drosophila, homolog of, 7
• Frizzled7
• Frizzled-7
• fz-7
• FZD7
• FzE3frizzled-7
• hFz7

Background

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1‑3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1‑32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44‑169), a variable-length linker region (aa 170‑256), a seven-pass transmembrane region (aa 257‑549), and a variable-length C-terminal cytoplasmic domain (aa 550‑574) (1‑3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33‑185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta ‑Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3‑5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).

1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
4. Medina, A. et al. (2000) Mech. Dev. 92:227.
5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
7. Melchior, K. et al. (2008) Biol. Chem. 389:897.
8. Vincan, E. et al. (2007) Oncogene 26:2340.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-7 Antibody (MAB1981)(6)

Publications using MAB1981

• Hu, DJ;Cai, XT;Simons, J;Yun, J;Elstrott, J;Jasper, H; Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway Nature communications 2025-05-03 [PMID: 40319020] (Immunohistochemistry, Transgenic Mouse)
• Alfredo Erazo-Oliveras, Mónica Muñoz-Vega, Mohamed Mlih, Venkataramana Thiriveedi, Michael L. Salinas, Jaileen M. Rivera-Rodríguez et al. Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic beta -catenin Nature Communications 7/19/2023 [PMID: 37468468]
• E Flores-Her, DM Velázquez, MC Castañeda-, G Fuentes-Ga, G Fonseca-Ca, JK Yamamoto-F, MT Romero-Avi, JA García-Sái, M Robles-Flo Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells Cell. Signal., 2020-04-10;72(0):109636. 2020-04-10 [PMID: 32283254] (Flow Cytometry, Human)
• Wu DT, Seita Y, Zhang X Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells. PLoS ONE, 2012-04-20;7(4):e34778. 2012-04-20 [PMID: 22536330] (Flow Cytometry, Human)
• Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells. Cell Stem Cell, 2009-03-06;4(3):248-62. 2009-03-06 [PMID: 19265664] (Flow Cytometry, Human)
• Baksh D, Boland GM, Tuan RS Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. J. Cell. Biochem., 2007-08-01;101(5):1109-24. 2007-08-01 [PMID: 17546602] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: FZD7
• Uniprot:
  • Q61090()